LOCUS       beta-lactamase;        5932 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pZ1918 with lacZ cassette, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    beta-lactamase; beta-galactosidase
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5932)
  AUTHORS   Schweizer HP.
  TITLE     Two plasmids, X1918 and Z1918, for easy recovery of the xylE and 
            lacZ reporter genes
  JOURNAL   Gene 134 (1), 89-91 (1993)
  PUBMED    8244036
REFERENCE   2  (bases 1 to 5932)
  AUTHORS   Su TZ, Schweizer H, Oxender DL.
  TITLE     A novel phosphate-regulated expression vector in Escherichia coli
  JOURNAL   Gene 90 (1), 129-133 (1990)
  PUBMED    2379833
REFERENCE   3  (bases 458 to 532)
  AUTHORS   Simons RW, Houman F, Kleckner N.
  TITLE     Improved single and multicopy lac-based cloning vectors for protein 
            and operon fusions
  JOURNAL   Gene 53 (1), 85-96 (1987)
  PUBMED    3596251
REFERENCE   4  (bases 3619 to 3647)
  AUTHORS   Casadaban MJ, Martinez-Arias A, Shapira SK, Chou J.
  TITLE     Beta-galactosidase gene fusions for analyzing gene expression in 
            escherichia coli and yeast
  JOURNAL   Meth. Enzymol. 100, 293-308 (1983)
  PUBMED    6312261
REFERENCE   5  (bases 1 to 5932)
  AUTHORS   Schweizer HP.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-1993) Herbert P. Schweizer, Microbiology and 
            Infectious Diseases, University of Calgary, 3330 Hospital Drive 
            N.W., Calgary, Alberta, T2N 4N1, Canada
REFERENCE   6  (bases 1 to 5932)
  TITLE     Direct Submission
REFERENCE   7  (bases 1 to 5932)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1993"; volume: "134"; issue: "1"; pages: "89-91"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Gene"; 
            date: "1990"; volume: "90"; issue: "1"; pages: "129-133"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Gene"; 
            date: "1987"; volume: "53"; issue: "1"; pages: "85-96"
COMMENT     SGRef: number: 4; type: "Journal Article"; journalName: "Meth. 
            Enzymol. 100, 293-308 (1983)"
COMMENT     SGRef: number: 5; type: "Journal Article"; journalName: "Submitted 
            (03-DEC-1993) Herbert P. Schweizer, Microbiology and Infectious 
            Diseases, University of Calgary, 3330 Hospital Drive N.W., Calgary, 
            Alberta, T2N 4N1, Canada"
COMMENT     SGRef: number: 6; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5932
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(399..454)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     misc_feature    458..532
                     /label=Escherichia coli trp operon DNA
                     /note="Escherichia coli trp operon DNA"
                     /citation=[3]
     regulatory      539..542
                     /gene="lacZ"
                     /regulatory_class="ribosome_binding_site"
     CDS             550..3621
                     /label=lacZ
                     /note="beta-galactosidase"
     misc_feature    3619..3647
                     /label=junction between lac operon DNA and polylinker
                     /note="junction between lac operon DNA and polylinker"
                     /citation=[4]
     misc_feature    3643..3698
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(3711..3727)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3735..3751)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3759..3789)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3804..3825)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4113..4701)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4875..5732)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5733..5837)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg
      421 actctagagg atccccgggt accgagctcg gatccggaca accgatgaaa gcggcgacgc
      481 gcagttaatc ccacagccgc cagttccgct ggcggcattt taactttctt tatcacacag
      541 gaaacagcta tgaccatgat tacggattca ctggccgtcg ttttacaacg tcgtgactgg
      601 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
      661 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
      721 gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtgc
      781 gatcttcctg aggccgatac tgtcgtcgtc ccctcaaact ggcagatgca cggttacgat
      841 gcgcccatct acaccaacgt aacctatccc attacggtca atccgccgtt tgttcccacg
      901 gagaatccga cgggttgtta ctcgctcaca tttaatgttg atgaaagctg gctacaggaa
      961 ggccagacgc gaattatttt tgatggcgtt aactcggcgt ttcatctgtg gtgcaacggg
     1021 cgctgggtcg gttacggcca ggacagtcgt ttgccgtctg aatttgacct gagcgcattt
     1081 ttacgcgccg gagaaaaccg cctcgcggtg atggtgctgc gttggagtga cggcagttat
     1141 ctggaagatc aggatatgtg gcggatgagc ggcattttcc gtgacgtctc gttgctgcat
     1201 aaaccgacta cacaaatcag cgatttccat gttgccactc gctttaatga tgatttcagc
     1261 cgcgctgtac tggaggctga agttcagatg tgcggcgagt tgcgtgacta cctacgggta
     1321 acagtttctt tatggcaggg tgaaacgcag gtcgccagcg gcaccgcgcc tttcggcggt
     1381 gaaattatcg atgagcgtgg tggttatgcc gatcgcgtca cactacgtct gaacgtcgaa
     1441 aacccgaaac tgtggagcgc cgaaatcccg aatctctatc gtgcggtggt tgaactgcac
     1501 accgccgacg gcacgctgat tgaagcagaa gcctgcgatg tcggtttccg cgaggtgcgg
     1561 attgaaaatg gtctgctgct gctgaacggc aagccgttgc tgattcgagg cgttaaccgt
     1621 cacgagcatc atcctctgca tggtcaggtc atggatgagc agacgatggt gcaggatatc
     1681 ctgctgatga agcagaacaa ctttaacgcc gtgcgctgtt cgcattatcc gaaccatccg
     1741 ctgtggtaca cgctgtgcga ccgctacggc ctgtatgtgg tggatgaagc caatattgaa
     1801 acccacggca tggtgccaat gaatcgtctg accgatgatc cgcgctggct accggcgatg
     1861 agcgaacgcg taacgcgaat ggtgcagcgc gatcgtaatc acccgagtgt gatcatctgg
     1921 tcgctgggga atgaatcagg ccacggcgct aatcacgacg cgctgtatcg ctggatcaaa
     1981 tctgtcgatc cttcccgccc ggtgcagtat gaaggcggcg gagccgacac cacggccacc
     2041 gatattattt gcccgatgta cgcgcgcgtg gatgaagacc agcccttccc ggctgtgccg
     2101 aaatggtcca tcaaaaaatg gctttcgcta cctggagaga cgcgcccgct gatcctttgc
     2161 gaatacgccc acgcgatggg taacagtctt ggcggtttcg ctaaatactg gcaggcgttt
     2221 cgtcagtatc cccgtttaca gggcggcttc gtctgggact gggtggatca gtcgctgatt
     2281 aaatatgatg aaaacggcaa cccgtggtcg gcttacggcg gtgattttgg cgatacgccg
     2341 aacgatcgcc agttctgtat gaacggtctg gtctttgccg accgcacgcc gcatccagcg
     2401 ctgacggaag caaaacacca gcagcagttt ttccagttcc gtttatccgg gcaaaccatc
     2461 gaagtgacca gcgaatacct gttccgtcat agcgataacg agctcctgca ctggatggtg
     2521 gcgctggatg gtaagccgct ggcaagcggt gaagtgcctc tggatgtcgc tccacaaggt
     2581 aaacagttga ttgaactgcc tgaactaccg cagccggaga gcgccgggca actctggctc
     2641 acagtacgcg tagtgcaacc gaacgcgacc gcatggtcag aagccgggca catcagcgcc
     2701 tggcagcagt ggcgtctggc ggaaaacctc agtgtgacgc tccccgccgc gtcccacgcc
     2761 atcccgcatc tgaccaccag cgaaatggat ttttgcatcg agctgggtaa taagcgttgg
     2821 caatttaacc gccagtcagg ctttctttca cagatgtgga ttggcgataa aaaacaactg
     2881 ctgacgccgc tgcgcgatca gttcacccgt gcaccgctgg ataacgacat tggcgtaagt
     2941 gaagcgaccc gcattgaccc taacgcctgg gtcgaacgct ggaaggcggc gggccattac
     3001 caggccgaag cagcgttgtt gcagtgcacg gcagatacac ttgctgatgc ggtgctgatt
     3061 acgaccgctc acgcgtggca gcatcagggg aaaaccttat ttatcagccg gaaaacctac
     3121 cggattgatg gtagtggtca aatggcgatt accgttgatg ttgaagtggc gagcgataca
     3181 ccgcatccgg cgcggattgg cctgaactgc cagctggcgc aggtagcaga gcgggtaaac
     3241 tggctcggat tagggccgca agaaaactat cccgaccgcc ttactgccgc ctgttttgac
     3301 cgctgggatc tgccattgtc agacatgtat accccgtacg tcttcccgag cgaaaacggt
     3361 ctgcgctgcg ggacgcgcga attgaattat ggcccacacc agtggcgcgg cgacttccag
     3421 ttcaacatca gccgctacag tcaacagcaa ctgatggaaa ccagccatcg ccatctgctg
     3481 cacgcggaag aaggcacatg gctgaatatc gacggtttcc atatggggat tggtggcgac
     3541 gactcctgga gcccgtcagt atcggcggaa ttccagctga gcgccggtcg ctaccattac
     3601 cagttggtct ggtgtcaaaa ataataataa ccgggcaggg gggatccgag ctcggtaccc
     3661 ggggatcctc tagagtcgac ctgcaggcat gcaagcttgg cgtaatcatg gtcatagctg
     3721 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     3781 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     3841 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     3901 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     3961 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     4021 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     4081 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     4141 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     4201 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     4261 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     4321 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     4381 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     4441 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     4501 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     4561 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     4621 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     4681 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     4741 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     4801 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     4861 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     4921 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     4981 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     5041 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     5101 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     5161 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     5221 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     5281 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     5341 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     5401 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     5461 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     5521 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     5581 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     5641 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     5701 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     5761 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     5821 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     5881 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tc
//
